DNA

Part:BBa_J100137

Designed by: Catherine Doyle   Group: Campbell M Lab   (2014-04-16)


Theophylline Riboswitch 1

This part combines Theophylline Riboswitch 1 (Part J100137) and superfolder GFP optimized for E. coli (Part J100070). It was assembled using the Golden Gate assembly Method and the BsaI sites that were engineered into the riboswitch and the superfolder GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3083
    Illegal suffix found in sequence at 877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3083
    Illegal SpeI site found at 878
    Illegal PstI site found at 892
    Illegal NotI site found at 885
    Illegal NotI site found at 3089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3083
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3083
    Illegal suffix found in sequence at 878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3083
    Illegal XbaI site found at 3098
    Illegal SpeI site found at 878
    Illegal PstI site found at 892
    Illegal AgeI site found at 100
    Illegal AgeI site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 169


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